Phase contrast device PKU-2
Phase-contrast microscopy is a method of microscopic examination based on obtaining, using special devices, a contrast image of structures of colorless transparent microobjects that differ in density (for example, living microorganisms and tissue cultures). When a beam of light passes through an unpainted object, only the oscillation phase of the light wave changes, which is not perceived by the human eye. In order for the image to become contrasty, it is necessary to convert the phase changes of the light wave into visible amplitude ones. This is achieved using a phase contrast condenser and a phase lens. A phase contrast condenser is a lens with a revolver and a set of annular diaphragms for each lens. The phase lens is equipped with a phase plate, which is obtained by applying salts of rare earth elements to the lens. The image of the annular diaphragm coincides with the ring of the phase plate of the corresponding lens. Phase-contrast microscopy significantly increases the contrast of the object and is used mainly for the study of native preparations. Manba: https://www.nv-lab.ru/catalog_info.php?ID=601